Shavit Lab

We study the genetics of blood clotting and bleeding using zebrafish as a model. Our lab has generated approximately 10 lines of fish with mutations in different blood coagulation genes known to be associated with bleeding disorders in humans using Zinc finger nucleases, Talens, and CRISPR technology. For each mutation we identify any phenotypes related to blood clotting and bleeding and attempt to rescue lethal mutations. We regularly genotype our fish using PCR and gel electrophoresis. To reduce our hazardous waste, we recently acquired a Qiaxcel that allows us to determine the size of bands without using ethidium bromide gels. We replaced our older -80 freezer with a newer more energy efficient model and turned it up to -70. {Previously certified gold: August 11, 2017}